Synthesis of Murine Leukemia Virus Proteins Differentiating Friend Erythroleukemia Cells1

نویسندگان

  • Corinne C. Sherton
  • Steven L. Dresler
  • Ethel Polonoff
  • Michael C. Webb
  • David Kabat
چکیده

In agreement with previous observations, the Friend 745 line of virus-induced erythroleukemia cells can be induced by dimethyl sulfoxide to synthesize hemoglobin and to increase simultaneously their content of morpho logically recognizable virus particles, especially in cytoplasmic vesicles. In apparent contrast to the electron microscopic results, the cellular content of the major murine leukemia virus (MuLV) proteins p30 (the major internal core protein) and gp70 (the envelope glycoprotein) were measured by radioimmunoassay and were found to be approximately the same in control and in induced cells. Control and induced cells are also approxi mately as active in the synthesis of the protein precursors of p30, reverse transcriptase, and gp70 in the processing of these precursors by proteolysis and glycosylation. Fur thermore, the noninfectious MuLV synthesized by this cell line is released into the culture medium to the same extent by control and induced cells. Although some of the virions formed in induced Friend 745 cells bud into cytoplasmic vesicles that are absent from control cells, this intravesicular budding is not associated with a major increase in the synthesis, processing, or cellular content of virion proteins. MuLV synthesis by Friend 745 cells was compared with that of Ostertag-derived F46/K erythroleukemia cells that produce infectious virus. The synthesis and processing of MuLV-specific proteins were very similar in these inde pendently isolated cell lines, despite the major difference in infectivity of the virus produced.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Synthesis of murine leukemia virus proteins differentiating Friend erythroleukemia cells.

In agreement with previous observations, the Friend 745 line of virus-induced erythroleukemia cells can be induced by dimethyl sulfoxide to synthesize hemoglobin and to increase simultaneously their content of morpho logically recognizable virus particles, especially in cytoplasmic vesicles. In apparent contrast to the electron microscopic results, the cellular content of the major murine leuke...

متن کامل

Studies of cloned Friend erythroleukemia tumor cells. Modulation of the tumor-specific cytologic T lymphocyte response by infectious Friend virus production in vitro

The HFL/b tumor cell line, induced by Friend erythroleukemia virus in BALB.B mice, was used to study the relation between virus production or nonproduction and the antigens recognized by Friend virus-specific cytolytic T lymphocytes (CTL). Analysis of clones and subclones of these tumor cells revealed a high degree of heterogeneity with respect to the production and release into culture fluids ...

متن کامل

Changes in DMA Associated with Induction of Erythroid Differentiation by Dimethyl Sulfoxide in Murine Erythroleukemia Cells1

The Friend virus-infected murine erythroleukemia cell can be induced to differentiate along erythroid cells in culture with various compounds, including dimethyl sulfoxide. DNA from murine erythroleukemia cells cultured with dimethyl sulfoxide shows a decrease in sedimenta tion rate in alkaline sucrose gradients after alkali lysis of the cells. These changes can be detected as early as 27 hr af...

متن کامل

Spontaneous regression of Friend virus-induced erythroleukemia. I. The role of the helper murine leukemia virus component

The RFV strain of the Friend virus complex induces an erythroleukemia that spontaneously regresses. The tropism of regressing Friend virus complex (RFV), which is conferred by its helper MuLV component, MuLV-RF, is different from that of the conventional virus strain, CFV. RFV is NB-tropic and CFV is N-tropic. Passage of nonregressing CFV through Fv-1 incompatible Swiss/ICR mice changed the tro...

متن کامل

New cell surface gp70 related to Friend mink cell focus-inducing virus is expressed on Friend virus-induced erythroleukemic spleen cells after elimination of ecotropic Friend virus gp70 in Rfv-3r/s mice

Spleen cells from Rfv-3r/s mice with Friend virus-induced erythroleukemia were analyzed for expression of virus-induced proteins with monoclonal antiviral antibodies and conventional antisera. Leukemic spleen cells, 30-60 d after virus inoculation, expressed decreased amounts of ecotropic Friend murine leukemia helper virus gag- and env-encoded cell surface and intracellular proteins compared w...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2006